“This is a pretty new disinfection technology, and we want to demonstrate in the small scale first, and then improve its real-world applications for point-of-use or off-grid water purification,” said Xing Xie, the Carlton S. Wilder Assistant Professor in the School of Civil and Environmental Engineering.
Xie and his postdoctoral researcher Ting Wang published the paper, “Nanosecond Bacteria Inactivation Realized by Locally Enhanced Electric Field Treatment,” in Nature Water in January.
Although conventional electric field treatment (CEFT) is applied for food pasteurization, it hasn’t been widely used for drinking water disinfection because of the relatively high cost. When water and bacteria are exposed to electricity, the bacteria cell membrane acts like a capacitor in a circuit. Typically, in CEFT, water’s low conductivity means nanosecond pulses won’t charge the membrane fast enough to kill bacteria.
The researchers created a locally enhanced electric field (LEEFT) that brought the electricity directly to the bacteria. The electrodes have gold nanotips that build up concentrated charges instantly when connected to electricity, enabling the charges to travel to the membrane and kill the bacteria much faster.
“This ultra-fast bacteria inactivation just using the nanosecond pulses is a surprise because, theoretically, nanosecond pulses are just too short to kill the bacteria in conventional electric field treatment because the membrane takes time to charge,” Wang said. “But with LEEFT’s nanowedges and nanostructures, the bacteria cells can be charged directly by the nanometal, quickly disinfecting water."
To test the technology, they fabricated gold nanowedges on the electrode edge of a chip. Then they added model bacteria Staphylococcus to the chip, a commonly used bacteria in labs and often found in water systems. Next, they applied electric pulses and watched how the bacteria reacted in real time under a microscope.
When electricity was applied at 40 kilovolts per centimeter for 200 nanoseconds, 95% of nanowedges successfully killed the bacteria. Compared to conventional EFT, LEEFT lowers applied eclectic field strength by eight times — and shortens the treatment time by 1 million times.
“We found that even nanosecond pulses could kill the bacteria in the LEEFT and not other circumstances,” Wang said. This near-instantaneous decontamination reduces how much electricity is needed to disinfect water, making this an affordable sanitation option and pointing to a future in which producing clean water may take less of a toll on the environment.
MEDICA-tradefair.com; Source: Georgia Institute of Technology
