Test for the detection of coronavirus SARS-CoV-1/2 (COVID-19)
A new severe respiratory syndrome caused by a coronavirus was first observed in Wuhan (China) in December 2019. The infection has spread all over the world, and consequently, on March 2020, the disease was declared a pandemic by the WHO. The new virus was called SARS-CoV-2, causing COVID-19 disease.
Most infected people with SARS-CoV-2 will develop mild to moderate symptoms, as fever, tiredness or dry cough, but it can also cause severe illness, and even death, especially in people with previous conditions and in elderly people. These symptoms may appear 2-14 days after exposure to the virus.
Detection of the RNA of the virus by techniques as RT-PCR real time is important in early infection because it can helps us to isolate infected people and contain the outbreak. According to the guidelines of the WHO(1), in those areas where the SARS-CoV-2 virus is widely spread, the detection of only one target of the virus by RT-PCR provides a simpler and easier diagnosis, with no need of an algorithm for the interpretation of results. In areas with low incidence of COVID-19, one target detection can also be used, although the result has to be confirmed by sequencing. The E gene provides the higher sensitivity for the detection of SARS viruses using only one target, as needed in the current situation in most countries with high COVID-19 prevalence. As at present no other SARS-like coronaviruses are circulating in the human population, the possibility of false positive result could be excluded.The Real SARS-CoV-1/2 kit is a kit for easy and sensitive detection of E gen of SARS-CoV-2 by RT-PCR real time using RNA extracted from human clinical samples.
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